Journal: The Journal of Cell Biology

Article Title: The 193-Kd Vault Protein, Vparp, Is a Novel Poly(Adp-Ribose) Polymerase

doi:

Figure Lengend Snippet: Tissue-specific expression patterns of p193 and MVP are similar. A Northern blot (Origene) containing poly(A) + RNA from human tissues was hybridized with either a 32 P-labeled probe specific for p193 (top), MVP (middle), or β-actin (bottom) as described in Materials and Methods. The tissue sources are indicated at the top of the blot.

Article Snippet: A multiple tissue Northern blot containing 2 μg of poly(A) + human RNA was purchased from Origene and hybridized following their protocol with a randomly primed p193 probe (bases 385–880).

Techniques: Expressing, Northern Blot, Labeling

Journal: PLoS ONE

Article Title: Identification and Characterization of Sulfated Carbohydrate-Binding Protein from Lactobacillus reuteri

doi: 10.1371/journal.pone.0083703

Figure Lengend Snippet: Supernatant, cell surface, and whole cell lysate fractions at different culture times were analyzed by western blotting. Outer surface proteins of L. reuteri JCM1081 were extracted with GHCl. Reactivity with anti-EF-Tu and anti-RNA polymerase antibodies is shown. An anti-RNA polymerase antibody was used to confirm whether cell lysis occurred. Culture time and pH are indicated in the table.

Article Snippet: Anti-RNA polymerase antibodies (1∶2500 dilution; Acris Antibodies, San Diego, CA, USA) were used to verify that cell lysis had occurred.

Techniques: Western Blot, Lysis